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Substrates Used by Brochothrix thermosphacta When Growing on Meat.

Frederick H Grau1

  • 1CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland 4170, Australia.

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Brochothrix thermosphacta utilizes glucose and ribose for anaerobic growth on meat, with ribose likely being the primary energy source. Aerobic growth involves a broader range of substrates including glycerol and inosine.

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Area of Science:

  • Food microbiology
  • Bacterial metabolism

Background:

  • Brochothrix thermosphacta is a common spoilage bacterium in refrigerated meat products.
  • Understanding its metabolic capabilities is crucial for predicting spoilage and developing control strategies.

Purpose of the Study:

  • To identify the specific carbon substrates utilized by Brochothrix thermosphacta under aerobic and anaerobic growth conditions on meat.
  • To determine the primary energy source for anaerobic growth, particularly in high pH environments.

Main Methods:

  • Growth experiments using Brochothrix thermosphacta on meat-based media.
  • Analysis of substrate utilization patterns under both aerobic and anaerobic conditions.

Main Results:

  • Aerobic growth utilized glucose, ribose, glycerol, glycerol-3-phosphate, and inosine.
  • Glycogen and inosine-monophosphate were not utilized under aerobic conditions.
  • Anaerobic growth was limited to the metabolism of glucose and ribose.
  • Ribose was identified as the probable major energy source for anaerobic growth in high pH meat.

Conclusions:

  • Brochothrix thermosphacta exhibits distinct substrate utilization profiles depending on oxygen availability.
  • Ribose plays a critical role in the anaerobic metabolism of this meat spoilage bacterium.