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Related Experiment Videos

Alternative splicing generates two distinct Eip28/29 gene transcripts in Drosophila Kc cells.

R A Schulz, L Cherbas, P Cherbas

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1986
    PubMed
    Summary

    The Drosophila Eip28/29 gene produces two polypeptides, EIP 28III and EIP 29III. A minor transcript, generated by alternative splicing, results in a short-form product whose abundance is not regulated by ecdysone.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Developmental Biology

    Background:

    • The Drosophila Eip28/29 gene encodes ecdysone-inducible polypeptides (EIP) 28III and 29III.
    • Ecdysone treatment of Kc cell lines rapidly increases Eip28/29 transcripts and EIP synthesis.

    Purpose of the Study:

    • To characterize a minor transcript of the Eip28/29 gene.
    • To investigate the regulatory mechanisms of Eip28/29 gene expression.

    Main Methods:

    • Analysis of Eip28/29 gene and transcript sequences.
    • Identification and characterization of alternative splicing events.

    Main Results:

    • A minor transcript, approximately 25% of total Eip28/29 transcripts, was identified in both untreated and ecdysone-treated cells.

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  • This minor transcript arises from the use of an alternative splice donor site.
  • The short-form translation product lacks an internal dibasic tetrapeptide compared to the long form.

    • Conclusions:

    • The relative abundance of EIP 28/29 products is not hormonally regulated.
    • The long and short forms likely represent distinct mRNAs for EIP 28III and EIP 29III, respectively.