Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunohistologic analysis of human renal allograft dysfunction.

W C Waltzer, F Miller, A Arnold

    Transplantation
    |January 1, 1987
    PubMed
    Summary

    Percutaneous core needle biopsy aids in diagnosing renal allograft dysfunction by identifying infiltrating immune cells. This method helps differentiate rejection from other causes, guiding treatment and improving patient outcomes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The impact of musculoskeletal ill health on quality of life and function after critical care: a multicentre prospective cohort study.

    Anaesthesia·2024
    Same author

    [Telemedical aftercare of patients with skin diseases].

    Dermatologie (Heidelberg, Germany)·2022
    Same author

    Childhood maltreatment is not associated with atopic dermatitis in adults: results from a cross-sectional population-based cohort study.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2022
    Same author

    Nosocomial or not? A combined epidemiological and genomic investigation to understand hospital-acquired COVID-19 infection on an elderly care ward.

    Infection prevention in practice·2021
    Same author

    A method to assess heart rate variability in neonate rats: validation in normotensive and hypertensive animals.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2020
    Same author

    Severe COVID-19 and healthcare-associated infections on the ICU: time to remember the basics?

    The Journal of hospital infection·2020

    Area of Science:

    • Nephrology
    • Immunology
    • Transplantation

    Background:

    • Renal allograft dysfunction diagnosis relies on understanding intragraft immune responses.
    • Immunohistology offers detailed cellular phenotyping of infiltrating cells.

    Purpose of the Study:

    • To evaluate the utility of percutaneous core needle biopsy in immunohistological assessment of renal allograft dysfunction.
    • To identify specific immune cell phenotypes associated with different causes of graft dysfunction.

    Main Methods:

    • 72 percutaneous core needle biopsies from 42 renal transplant patients were analyzed.
    • Immunohistochemistry with monoclonal antibodies and immunoperoxidase staining identified cell phenotypes.
    • Light microscopy, electron microscopy, and immunofluorescence were also performed.

    Related Experiment Videos

    Main Results:

    • Acute interstitial rejection showed increased pan-T cells (Leu 1), cytotoxic/suppressor cells (Leu 2), and helper T cells (Leu 3).
    • DR antigen expression was elevated in both acute and chronic rejection compared to acute tubular necrosis.
    • Biopsy results contradicted clinical diagnoses in 38% of cases, influencing steroid treatment decisions.

    Conclusions:

    • Percutaneous core needle biopsy is valuable for studying intragraft immune events in renal allografts.
    • Immunohistological evaluation guides clinical management and differentiates rejection from other causes of dysfunction.
    • Severe small vessel disease and antibody-mediated rejection correlate with allograft loss.