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STIM1 expression is associated with osteosarcoma cell survival

Jie Zang1,2, Dongqing Zuo1,3, Kristen L Shogren1

  • 1Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.

Chinese Journal of Cancer Research = Chung-Kuo Yen Cheng Yen Chiu
|April 19, 2019
PubMed
Abstract

Insights

Stromal interaction molecule 1 (STIM1) is crucial for osteosarcoma cell survival and migration. Blocking store-operated calcium entry (SOCE) and STIM1 inhibits osteosarcoma progression, highlighting their potential as therapeutic targets.

Area of Science:

  • Oncology
  • Cell Biology
  • Calcium Signaling

Background:

  • Osteosarcoma is a primary bone malignancy with limited treatment options.
  • Store-operated calcium entry (SOCE) and Stromal Interaction Molecule 1 (STIM1) are implicated in cancer cell functions.
  • Understanding their role in osteosarcoma is critical for developing new therapies.

Purpose of the Study:

  • To investigate the role of SOCE and STIM1 in osteosarcoma cell survival and migration.
  • To determine the effect of blocking SOCE and STIM1 on osteosarcoma progression.
  • To explore the downstream signaling pathways regulated by SOCE in osteosarcoma.

Main Methods:

  • Western blot and immunohistochemistry to assess STIM1 expression in osteosarcoma cells and tissues.
  • MTS assays and colony formation assays to evaluate cell viability and proliferation.
  • Wound healing and Boyden chamber assays to analyze cell migration.
  • Luciferase assays to measure NFATc1 activity.

Main Results:

  • STIM1 was overexpressed in osteosarcoma cell lines and patient tissues, correlating with poor survival.
  • Inhibition of SOCE and STIM1 significantly reduced osteosarcoma cell viability and migration.
  • Blocking store-operated calcium channels led to the down-regulation of NFATc1 in osteosarcoma cells.

Conclusions:

  • STIM1 plays an essential role in the survival and migration of osteosarcoma cells.
  • The STIM1 and SOCE pathway represents a promising molecular target for osteosarcoma treatment.
  • Targeting calcium entry could offer a novel therapeutic strategy for osteosarcoma.
Keywords:
CalciumNFATc1SOCESTIM1osteosarcoma

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