Related Experiment Video
Updated: Jan 26, 2026

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells
Published on: September 8, 2010
A Structural Perspective on the Regulation of Human Single-Stranded DNA Binding Protein 1 (hSSB1, OBFC2B) Function in
Teegan Lawson1, Serene El-Kamand1, Ruvini Kariawasam1
1School of Science and Health, Western Sydney University, Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
Abstract:
Single-stranded DNA binding (SSB) proteins are essential to protect singe-stranded DNA (ssDNA) that exists as a result of several important DNA repair pathways in living cells. In humans, besides the well-characterised Replication Protein A (RPA) we have described another SSB termed human SSB1 (hSSB1, OBFC2B) and have shown that this protein is an important player in the maintenance of the genome. In this review we define the structural and biophysical details of how hSSB1 interacts with both DNA and other essential proteins. While the presence of the oligonucleotide/oligosaccharide (OB) domain ensures ssDNA binding by hSSB1, it has also been shown to self-oligomerise as well as interact with and being modified by several proteins highlighting the versatility that hSSB1 displays in the context of DNA repair. A detailed structural understanding of these processes will likely lead to the designs of tailored hSSB1 inhibitors as anti-cancer drugs in the near future.
More Related Videos
Related Concept Videos
Single-Strand DNA Binding Proteins
Overview of DNA Repair
Chemically...
Overview of DNA Repair
From DNA to Protein
Cooperative Binding of Transcription Regulators
DNA Helicases

