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Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

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When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
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Rapidly Varying Flow01:24

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Urine Studies II: Urine Culture and Sensitivity Test01:26

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Related Experiment Video

Updated: Jan 26, 2026

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila
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A rapid and sensitive enzymatic assay for 2,3-butanediol.

Gyu Bi Lee1,2, Yun Jae Kim1,2, Jae Kyu Lim1,2

  • 11Korea Institute of Ocean Science and Technology, Busan, 49111 Republic of Korea.

3 Biotech
|April 19, 2019
PubMed
Summary

A new enzymatic assay rapidly detects 2,3-butanediol (2,3-BDO) with high sensitivity. This method is 15 times more sensitive than HPLC, enabling efficient screening of 2,3-BDO-producing microbes.

Keywords:
2,3-Butanediol2,3-Butanediol dehydrogenaseEnzymatic assayHigh-throughput screening

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microbiology

Background:

  • Accurate detection of 2,3-butanediol (2,3-BDO) is crucial for identifying microbial production.
  • Existing methods like High-Performance Liquid Chromatography (HPLC) have limitations in sensitivity and throughput.

Observation:

  • A novel enzymatic assay was developed utilizing Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh).
  • The assay measures NADP+ reduction to quantify 2,3-BDO concentration.

Findings:

  • The enzymatic assay detects 2,3-BDO at concentrations as low as 0.01 mM.
  • This represents a 15-fold increase in sensitivity compared to HPLC, which requires >0.15 mM.
  • In high-throughput screening, the enzymatic assay identified 14 positive microbial samples, versus 8 by HPLC.

Implications:

  • The developed enzymatic assay offers a rapid, sensitive, and effective method for detecting 2,3-BDO.
  • It is suitable for high-throughput screening of 2,3-BDO-producing microorganisms in a microtiter plate format.
  • This advancement can accelerate research and development in bio-based chemical production.