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Published on: March 13, 2015
[Application and Evaluation of Chemiluminescence Immunoassay in Blood Screening]
Shu-Bo Lin1, Ze-Xuan Zheng2, Rui Zhang2
1Shantou Blood Center, Shantou 515064, Guangdong Province, China,E-mail: lsbtop@163.com.
Objective:
To explore the application of blood screening method based on chemiluminescence immunoassay(CLIA)and to evaluate its officacy.
Methods:
Screening HBsAg, anti-HCV, anti-HIV and TP was performed on 3,530 voluntary blood donors by ELISA and CLIA, and then all the specimens with ELISA- and ELISA+/CLIA- were further confirmed by NAT; TP single and double positive specimens by ELISA or CLIA were further confirmed by TPPA.
Results:
The results of CLIA method was well consistent with NAT results, displaying better repeatability and higher sensitivity than ELISA method. For CLIA+/ELISA- specimens there was a certain false-negative result obtained by ELISA method, especially for blood donors with low virus biter concentration or "window period".
Conclusion:
ELISA and CLIA have complementary advantages in blood screening, which can improve the sensitivity of blood screening, reduce the missed detection and shorten detection time. The introduction of CLIA for blood screening is of great importance for ensuring the quality of blood and the safety of clinical transfusion.
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