Related Experiment Video
Updated: Jan 26, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
Rapid quantitative analysis of ginkgo flavonoids using paper spray mass spectrometry
Wei Zhou1, Zihui Yang1, Si Huang1
1Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
Abstract:
In this work, a method for the rapid and reliable analysis of ginkgo flavonoids was developed by using paper spray mass spectrometry (PS-MS) assisted by microwave-assisted hydrolysis (MAH). Kaempferol-D4, quercetin-D5, and isorhamnetin-D3 were used as internal standards (IS). Samples analyses were completed by simply treating with extraction, MAH, dilution, and quantitative analysis by PS-MS. Because of the absence of HPLC separation, the PS-MS analysis time was less than 20 s. The linear ranges of kaempferol, quercetin and isorhamnetin were 1.7-86, 1.7-86, and 1.3-68 mg/L, respectively, with linear coefficients (R2) of 0.9993-0.9998. The detection limits (LODs) were 0.1, 0.1, and 0.2 mg/L. Limits of quantifications (LOQs) were all less than 0.6 mg/L. Compared with HPLC results, there are no obvious differences. It can be concluded that the PS-MS method with isotope IS is accurate for the rapid analysis of ginkgo flavonoids in extracts and related products.
More Related Videos
06:43Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
08:56Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry
Mass Spectrometry: Overview
Mass Spectrometry of Amines
Mass Spectrometry: Isotope Effect
MALDI-TOF Mass Spectrometry