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Updated: Jan 26, 2026

Use of Drosophila S2 Cells for Live Imaging of Cell Division
Published on: August 23, 2019
Plant cell division - defining and finding the sweet spot for cell plate insertion
1Center for Plant Molecular Biology, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
Abstract:
The plant microtubules form unique arrays using acentrosomal microtubule nucleation pathways, yet utilizing evolutionary conserved centrosomal proteins. In cytokinesis, a multi-component cytoskeletal apparatus, the phragmoplast mediates the biosynthesis of the new cell plate by dynamic centrifugal expansion, a process that demands exquisite coordination of microtubule turnover and endomembrane trafficking. At the same time, the phragmoplast is guided to meet with the parental wall at a cortical site that is predefined before mitotic entry and transiently marked by the preprophase band of microtubules. The cortical division zone maintains positional information of the selected division plane for the entire duration of cell division and for the guidance of the phragmoplast during cytokinesis. Its establishment is an essential requirement for normal plant organogenesis, due to the confinement of cells by rigid cell walls.
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