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Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Published on: November 6, 2021
Effect of sodium nitroprusside treatment on shikimate and phenylpropanoid pathways of apple fruit
Yonghong Ge1, Yanru Chen1, Canying Li1
1College of Food Science and Technology, Bohai University, 121013 Jinzhou, PR China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, 121013 Jinzhou, PR China.
Abstract:
Blue mould caused by Penicillium expansum is one of the important diseases of apple fruit during storage. Phenylpropanoid pathway is an important induction mechanism that can utilize downstream metabolites of shikimate pathway to synthesize a series of secondary metabolites. Apple fruit (cv. Fuji) were treated with sodium nitroprusside (SNP) to study its effect on blue mould, shikimate and phenylpropanoid pathways. The results showed that 1.0 mmol L-1 SNP significantly inhibited lesion development of apple fruit inoculated with P. expansum. The results also indicated that SNP enhanced MdDHQS, MdSKDH, MdSK and MdEPSPS genes expressions, increased shikimic acid, tryptophan, tyrosine and phenylalanine contents in apple fruit. The activities of phenylalanine ammonialyase, 4-coumarate: coenzyme A, ligase, cinnamate 4-hydroxylase, lignin, total phenolic compounds and flavonoids contents in apple fruit were also increased by SNP treatment. These results suggest that SNP might modulate shikimate and phenylpropanoid pathways to enhance disease resistance of apple fruit.
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