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High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica
Mingyue Piao1, Donglei Zou2, Xianghao Ren3
1Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China; College of Environmental Science and Engineering, Jilin Normal University, 1301 Haifeng Road, Siping 136000, China.
Abstract:
We developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Also, the SER/Lac presented better reusability compared to ADS/Lac, and it could be reused for three times without decrease of biotransformation efficiency, and half of it was remained after ten times use. Due to great stability and robustness property, SER/Lac was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.
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