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Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Milk Fat Globule-Epidermal Growth Factor-Factor 8 Reverses Lipopolysaccharide-Induced Microglial Oxidative Stress

Jie Li1, Xiaotian Xu2, Xiaoying Cai3

  • 1Department of Neurology, Zengcheng District People's Hospital of Guangzhou, Guangzhou, China.

Oxidative Medicine and Cellular Longevity
|April 20, 2019
PubMed

Insights

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8) protects against microglial oxidative stress in neuroinflammation. MFG-E8 reverses lipopolysaccharide-induced damage by regulating the Keap-1/Nrf-2/HO-1 pathway.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Immunology

Background:

  • Oxidative stress is implicated in neurological disorders.
  • Microglia play a key role in neuroinflammation.
  • The function of MFG-E8 in microglial oxidative stress is unknown.

Purpose of the Study:

  • To investigate the role of MFG-E8 in microglial oxidative stress.
  • To elucidate the underlying molecular mechanisms.

Main Methods:

  • In vitro and in vivo models of microglial oxidative stress induced by lipopolysaccharide (LPS).
  • Assessment of oxidative stress markers (TNF-α, IL-6, MDA, ROS, GSH, SOD).
  • Analysis of the Keap-1/Nrf-2/HO-1 pathway components.

Main Results:

  • LPS induced oxidative stress in mouse brains and primary microglia.
  • MFG-E8 pretreatment reversed LPS-induced oxidative stress markers.
  • MFG-E8 modulated the expression of Keap-1, Nrf-2, and HO-1.

Conclusions:

  • MFG-E8 exhibits antioxidant effects in neuroinflammation.
  • MFG-E8 may exert its protective effects via the Keap-1/Nrf-2/HO-1 pathway.

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