Related Experiment Video
Updated: Jan 26, 2026

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
Published on: June 16, 2023
Long noncoding RNA AFAP1-AS1 promoted osteosarcoma proliferation and invasion via upregulating BDNF
1Department of Orthopaedic Surgery, The No. 1 People's Hospital of Jingzhou, Jingzhou, China. cailin225@126.com.
Objective:
Recently, long noncoding RNAs (lncRNAs) have got much attention for their role in tumor progression. LncRNA AFAP1-AS1 was studied in this research to identify how it affects the development and proliferation of osteosarcoma.
Patients And Methods:
The mRNA expression of AFAP1-AS1 in osteosarcoma cells and tissue samples was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, cell proliferation assay and Matrigel assay were performed. Furthermore, the underlying mechanism was explored by using qRT-PCR and Western blot assay.
Results:
The expression level of AFAP1-AS1 was higher in osteosarcoma samples than that in adjacent tissues. In addition, the proliferation and invasion were inhibited after AFAP1-AS1 was downregulated in vitro. Besides, the mRNA and protein expression levels of brain derived neurotrophic factor (BDNF) were reduced after downregulation of AFAP1-AS1. Furthermore, the expression level of BDNF was positively related to the expression of AFAP1-AS1 in osteosarcoma tissues.
Conclusions:
AFAP1-AS1 could enhance the proliferation and invasion of osteosarcoma cells by upregulating BDNF, which might be a potential therapeutic target in osteosarcoma.
Related Concept Videos
The Eukaryotic Promoter Region
RNA Polymerase II Accessory Proteins
Abnormal Proliferation
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...
RNA Stability

