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The effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injury

T Bilecik1, F Karateke, H Elkan

  • 1Department of Surgery, Istinye University, Faculty of Medicine, VM Mersin Medical Park Hospital, Mersin, Turkey. karatekefaruk@gmail.com.

European Review for Medical and Pharmacological Sciences
|April 20, 2019
PubMed
Abstract

Insights

This study investigated Transient Receptor Potential Melastatin (TRPM) channels in liver injury. Verapamil treatment affected TRPM gene expression and liver cell damage in a rat model of hepatic ischemia-reperfusion.

Area of Science:

  • Physiology
  • Molecular Biology
  • Pharmacology

Background:

  • Transient Receptor Potential Melastatin (TRPM) channels are calcium and magnesium ion transporters.
  • TRPM subclasses TRPM2, TRPM6, TRPM7, and TRPM8 are notably expressed in liver cells.
  • Hepatic ischemia-reperfusion (I/R) is a critical condition affecting liver function.

Purpose of the Study:

  • To examine the effects of verapamil on TRPM2, TRPM6, TRPM7, and TRPM8 gene expression.
  • To evaluate histopathological alterations in the liver following I/R injury and verapamil treatment.
  • To investigate the role of specific TRPM channels in hepatic I/R injury.

Main Methods:

  • A rat model of hepatic ischemia-reperfusion was established.
  • Animals were divided into sham, verapamil, I/R, and I/R-verapamil groups.
  • Gene expression was quantified using Real-Time quantitative Polymerase Chain Reaction (RT-qPCR), and tissue damage was assessed via Hematoxylin and Eosin (HE) staining.

Main Results:

  • TRPM2, TRPM6, TRPM7, and TRPM8 gene expression significantly increased in I/R and verapamil-treated groups compared to the sham group.
  • Hepatocytes in the I/R group showed severe necrosis, degeneration, and hemorrhage.
  • The I/R-verapamil group exhibited moderate necrosis, degeneration, and hemorrhage.

Conclusions:

  • This study is the first to report on TRPM channel expression in a hepatic I/R rat model.
  • TRPM2, TRPM6, TRPM7, and TRPM8 play a role in the pathophysiology of hepatic ischemia-reperfusion.
  • Verapamil influences TRPM gene expression and liver histopathology during I/R injury.

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