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Updated: Jan 26, 2026

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
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Abatacept alleviates rheumatoid arthritis development by inhibiting migration of fibroblast-like synoviocytes via

Q-F Zou1, L Li, Q-R Han

  • 1Department of Rheumatology, Yantaishan Hospital, Yantai, China. uilao9352@163.com.

European Review for Medical and Pharmacological Sciences
|April 20, 2019
PubMed
Abstract

Insights

Abatacept inhibits rheumatoid arthritis (RA) progression by reducing fibroblast-like synoviocytes (FLS) migration and matrix metalloproteinase (MMP) expression through the mitogen-activated protein kinase (MAPK) pathway.

Area of Science:

  • Immunology
  • Cell Biology
  • Rheumatology

Background:

  • Rheumatoid arthritis (RA) is characterized by inflammation and joint destruction.
  • Fibroblast-like synoviocytes (FLS) play a key role in RA pathogenesis by migrating and producing matrix metalloproteinases (MMPs).
  • The mitogen-activated protein kinase (MAPK) pathway is implicated in regulating FLS activity.

Purpose of the Study:

  • To investigate Abatacept's effect on FLS migration and MMP expression.
  • To determine if Abatacept modulates the MAPK pathway in RA-FLS.
  • To elucidate the mechanism by which Abatacept influences RA progression.

Main Methods:

  • Quantitative Real-time-polymerase chain reaction (qRT-PCR) and ELISA were used to measure MMP1, MMP3, and MMP13 levels.
  • Western blot analysis assessed MAPK pathway activation.
  • Transwell assays evaluated FLS migratory capacity.

Main Results:

  • Abatacept significantly downregulated MMP1, MMP3, and MMP13 expression in RA-FLS.
  • Abatacept treatment inhibited FLS migration.
  • Abatacept downregulated MAPK pathway-related genes, and this effect was partially reversed by a MAPK inhibitor.

Conclusions:

  • Abatacept inhibits FLS migration and MMP expression by downregulating the MAPK pathway.
  • These actions suggest Abatacept can mitigate RA development.
  • Abatacept represents a potential therapeutic strategy for RA.

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