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Activation of P-TEFb by RBM7: To Live or Let Die

Nicolas Le May1, Frédéric Coin1

  • 1IGBMC (CNRS, INSERM, UdS), 1 rue Laurent Fries, 67400 Illkirch Graffenstaden, France.

Molecular Cell
|April 20, 2019
PubMed

Insights

Researchers discovered that RBM7 protein activates P-TEFb, a crucial step in the cell

Area of Science:

  • Molecular biology
  • Cellular response
  • Genetics

Background:

  • Cells possess intricate mechanisms to survive genotoxic stress.
  • Regulation of gene expression is critical for DNA damage response.
  • Promoter-proximal pausing of RNA polymerase II is a key regulatory step.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying the pro-survival response to genotoxic attack.
  • To identify key regulators involved in the activation of DNA damage response genes.

Main Methods:

  • Investigated the role of RBM7 in cellular response to genotoxic agents.
  • Assessed the activation of P-TEFb complex.
  • Analyzed RNA polymerase II release from promoter-proximal pausing.

Main Results:

  • Bugai et al. (2019) identified RBM7 as a crucial activator of P-TEFb.
  • RBM7-mediated P-TEFb activation leads to the release of RNA polymerase II from promoter-proximal pausing.
  • This release facilitates the expression of essential DNA damage response genes.

Conclusions:

  • RBM7-dependent activation of P-TEFb is a critical pro-survival mechanism.
  • This pathway is essential for mounting an effective transcriptional response to genotoxic stress.
  • Findings reveal a novel regulatory step in DNA damage repair pathways.

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