Related Experiment Video
Updated: Jan 26, 2026
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Published on: December 10, 2014
Comparison of the DNA Probe to Culture Methods for the Detection of Salmonella on Poultry Carcasses and Processing
A L Izat1, C D Driggers1, M Colberg1
1Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville, Arkansas 72701.
Abstract:
In a series of five experiments a total of 269 broiler carcass and chill tank water samples were tested for the presence of Salmonella using the DNA probe and the standard cultural method. Carcasses were sampled using a whole carcass rinse technique. Samples consisted of pre- (48) and post-chill (103) carcasses, and pre-chill (48) and chill (70) tank water. Samples to be evaluated with the DNA probe were subjected to three preenrichment/enrichment procedures to determine the most accurate and reliable enrichment procedure to use with the DNA probe assay. Direct enrichment in Selenite Cystine followed by 24 h incubation in Gram Negative broth allowed for recovery of 0.03 salmonellae/ml from carcass rinse and from pre-chill water using either the standard cultural method or the DNA probe. Preenrichment in Lactose broth produced inaccurate results for pre-chill carcass and pre-chill water samples using the DNA probe assay, and may be due to extreme microbiological competition. No false positive results were obtained using the DNA probe assay for any of the four sample types.
More Related Videos
12:50Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
08:24Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Related Concept Videos
03:31Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:08Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
02:57The Sense of Self: Reflected Self-Appraisal and Social Comparison
01:18The Scientific Method in Nursing Process
When using research findings to change practice, one must understand the process used to guide a study. The scientific method is a systematic, step-by-step process that supports the data's validity, reliability, and generalizability. As a result, findings can be...
01:23States of Water
Water freezes when the intermolecular forces are greater than the kinetic energy. Unlike most other substances, water is less dense in its solid state than in its liquid state. This is because each water molecule can form...
01:18Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...