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J T Peeler1, J W Messer1, G A Houghtby1
1Division of Microbiology, Food and Drug Administration, 1090 Tusculum Avenue, Cincinnati, Ohio 45226.
Abstract:
Inhibitory substance (antibiotic) test results from State Split Milk Samples were used to estimate precision parameters and to compare antibiotic medium 4 (A4) and PM indicator (PM) agars. Five inhibitory substances (ampicillin, cephapirin, erythromycin, neomycin, and penicillin-G) were tested. Repeatability relative standard deviations (RSDr) ranged from 1.0 to 4.8%, and the reproducibility relative standard deviations (RSDR) ranged from 4.8 to 10.4%. Zone sizes of erythromycin, neomycin, and penicillin-G were significantly larger on PM agar (α = 0.05) than on A4 agar. The reverse was observed for cephapirin. No difference between agars was noted for ampicillin.
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