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Related Experiment Video

Updated: Jan 26, 2026

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Published on: July 6, 2011

Morphometric analysis in mouse scleral fibroblasts using focused ion beam/scanning electron microscopy.

Kazuhisa Murata¹, Akira Hirata^2,3, Keisuke Ohta⁴

¹Department of Ophthalmology, Saga University Faculty of Medicine, Saga, Japan.

Scientific Reports

|April 21, 2019

Summary

This study reveals the 3D structure of mouse scleral fibroblasts using FIB/SEM. Scleral fibroblasts form cellular networks within collagenous layers, with posterior fibroblasts showing more complex arrangements.

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Last Updated: Jan 26, 2026

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

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Published on: July 6, 2011

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam

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Published on: July 26, 2014

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

Area of Science:

Ophthalmology
Cell Biology
Microscopy

Background:

The sclera and cornea form the eye's outer fibrous coat, crucial for protection and shape.
The precise 3D arrangement of scleral fibroblasts is not well understood.

Purpose of the Study:

To investigate the three-dimensional structure of mouse scleral fibroblasts.
To clarify the morphometric arrangement of these cells within the sclera.

Main Methods:

Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) was employed on mouse sclera.
Serial block face imaging and 3D reconstruction were performed using specialized software.
Scleral tissue was meticulously processed through fixation, dehydration, and resin embedding.

Main Results:

Scleral fibroblasts are organized in collagenous layers, forming interconnected cellular networks.
Cellular junctions were frequently observed between adjacent fibroblasts.
Posterior scleral fibroblasts exhibited a more intricate cellular arrangement compared to equatorial fibroblasts.

Conclusions:

The study provides novel insights into the 3D architecture of scleral fibroblasts.
Fibroblast arrangement varies between different scleral regions, particularly the posterior versus equatorial areas.
Understanding fibroblast organization is key for comprehending scleral biomechanics and ocular health.