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Updated: Jan 26, 2026

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Inhibition of 3β-Hydroxysteroid Dehydrogenase Type 1 Suppresses Interleukin-6 in Breast Cancer

Yuan-Ching Chang1, Chi-Hsin Lin2, Jiunn-Chang Lin1

  • 1Department of Surgery, MacKay Memorial Hospital and Mackay Medical College, Taipei, Taiwan; Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan; Mackay Junior College of Medicine, Nursing, and Management, Taipei, Taiwan.

The Journal of Surgical Research
|April 21, 2019
PubMed
Abstract

Insights

Targeting 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) in breast cancer may work by reducing interleukin-6 (IL-6). Inhibiting HSD3B1 suppressed tumor growth and enhanced chemotherapy effectiveness, partly through IL-6 suppression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Breast cancer patients with high HSD3B1 expression show poorer survival.
  • HSD3B1 inhibition reduces breast cancer cell growth and xenograft tumor development.
  • The precise mechanisms underlying HSD3B1's role in breast cancer remain unclear.

Purpose of the Study:

  • To investigate the molecular mechanisms by which HSD3B1 influences breast cancer progression.
  • To explore the relationship between HSD3B1, interleukin-6 (IL-6), and breast cancer cell behavior.
  • To assess the therapeutic potential of targeting HSD3B1 in breast cancer treatment.

Main Methods:

  • HSD3B1 was silenced in triple-negative breast cancer cell lines (MDA-MB-231, BT-20).
  • Microarray and bioinformatic analyses were employed to assess gene expression changes.
  • Interleukin-6 (IL-6) levels were quantified using qPCR and ELISA.
  • Clonogenic assays and cell viability assays were performed with and without IL-6 supplementation.

Main Results:

  • HSD3B1 silencing altered the expression of growth factors and cytokines.
  • Inhibition of HSD3B1 led to decreased IL-6 expression and secretion.
  • Reduced colony formation due to HSD3B1 inhibition was partially restored by IL-6.
  • HSD3B1 knockdown increased sensitivity to paclitaxel, an effect partially reversed by IL-6.

Conclusions:

  • The therapeutic benefits of targeting HSD3B1 in breast cancer are, in part, mediated by the suppression of IL-6.
  • IL-6 plays a significant role in mediating the effects of HSD3B1 on breast cancer cell growth and drug sensitivity.
Keywords:
3β-hydroxysteroid dehydrogenaseBreast cancerInterleukin-6Paclitaxel

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