Microelements in seminal and serum plasma are associated with fresh semen quality in Yorkshire boars

This study aimed to explore associations between semen quality and trace element level in serum and seminal plasma in Yorkshire boars. Semen quality of 112 Yorkshire boars was assessed for 13 weeks to calculate semen utilization rate, which was then divided into three categories: low utilization rate group (LG, < 60% utilization rate), medium utilization rate group (MG, 60-80%), and high utilization rate group (HG, > 80%). After grouping, serum and seminal plasma samples of selected boars were collected to determine concentrations of 10 elements including Ca, Mg, Cu, Fe, Zn, Mn, Se, Cr, Pb and Cd using inductively coupled plasma mass spectrometry. Results showed the increase of semen utilization rate was accompanied by the increase of sperm motility and the decrease of abnormal sperm rate among three groups (P < 0.01). Serum Fe concentration in LG boars was lower than that in HG boars (P < 0.05). Regression analysis revealed serum Fe concentration was positively correlated with sperm motility (r = 0.251; P < 0.05), while negatively correlated with abnormal sperm rate (r = -0.207; P < 0.05). However, MG and HG boars had lower serum Se concentration than LG boars (P < 0.05), and serum Se concentration contribution to sperm motility varied in a quadratic manner (Sperm motility = -0.0004 Se_(serum)² +0.136 Se+74.06; r = 0.300; P < 0.01). Semen utilization rate tended to decrease with the increase of seminal plasma Pb concentration (P = 0.09). Regression analysis exhibited seminal plasma Pb negatively related to sperm motility (r = -0.237; P < 0.05), while positively correlated with abnormal sperm rate (r = 0.237; P < 0.05). Furthermore, seminal plasma Pb was the most influential factor among trace element in serum and seminal plasma on sperm motility basing on the generalized linear model analysis (P < 0.05). Sperm motility decreased by approximately 3.47% when seminal plasma Pb concentration increased from 0 μg/L to 11.16 μg/L. In conclusion, deficiency of serum Fe reduces semen utilization rate by impairing sperm motility and morphology, whereas excessive serum Se decreases sperm motility. More importantly, the mere existence of seminal plasma Pb has more impact on semen quality than other trace elements in serum and seminal plasma in Yorkshire boars.