Related Experiment Video
Updated: Jan 26, 2026
10:59
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
10.2K
The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription
Olga Calvo1, Nathalie Grandin2, Antonio Jordán-Pla3
1Instituto de Biología Funcional y Genómica, CSIC-USAL, Salamanca, Spain.
Nucleic Acids Research
|April 23, 2019
Summary
The CST complex, including Ten1, regulates gene transcription by interacting with Spt5 and Hmo1. This suggests a role in coordinating transcription with DNA replication fork progression.
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- Specialized telomeric proteins are crucial for genome stability.
- The conserved CST complex (Cdc13, Stn1, Ten1) in yeast regulates telomere length and end protection.
- The function of CST beyond telomere maintenance is not fully understood.
Purpose of the Study:
- To investigate novel functions of the Ten1 protein, a component of the CST complex.
- To identify genetic and physical interactions of Ten1 with other cellular factors.
- To elucidate the role of CST in gene transcription regulation.
Main Methods:
- Genetic interaction analysis between TEN1 and transcription regulator genes.
- Molecular assays to measure RNA polymerase II and Spt5 occupancy.
- Co-immunoprecipitation assays to assess protein-protein interactions.
- Genome-wide analysis of gene expression in a ten1 mutant.
Main Results:
- Identified genetic interactions between TEN1 and transcription regulators.
- Demonstrated Ten1's role in regulating RNA polymerase II and Spt5 occupancy in transcribed genes.
- Showed physical association of CST complex members (Ten1, Cdc13, Stn1) with Spt5.
- Confirmed physical association between CST and Hmo1.
- Observed preferential Hmo1 binding at promoters of genes downregulated in the ten1 mutant.
Conclusions:
- Spt5 is a likely target of the CST complex in transcription regulation.
- CST, through Hmo1, may synchronize transcription with replication fork progression, particularly after head-on collisions.
- Ten1 and the CST complex have a novel role in regulating gene transcription and its coordination with DNA replication.
More Related Videos
09:26DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
4.8K
09:13Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
Published on: January 17, 2019
7.8K
Related Concept Videos
00:58Eukaryotic RNA Polymerases
26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
00:43Bacterial RNA Polymerase
32.6K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.6K
02:36RNA Polymerase II Accessory Proteins
10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K
02:41Telomeres and Telomerase
27.0K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
27.0K
02:23Master Transcription Regulators
7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
02:13Cooperative Binding of Transcription Regulators
7.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.2K