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Updated: Jan 26, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
Published on: April 28, 2022
Excitation parameters optimized for coherent anti-Stokes Raman scattering imaging of myelinated tissue
Craig Brideau1, Kelvin W C Poon1, Pina Colarusso2,3
1University of Calgary, Hotchkiss Brain Institute, Cumming School of Medicine, Department of Clinical, Canada.
Abstract:
Coherent anti-Stokes Raman scattering (CARS) generates a strong label-free signal in the long wavenumber C─H stretching region. Lipid-rich myelinated tissues, such as brain and spinal cord, would appear to be ideal subjects for imaging with CARS laser-scanning microscopy. However, the highly ordered, biochemically complex, and highly scattering nature of such tissues complicate the use of the technique. A CARS microscopy approach is presented that overcomes the challenges of imaging myelinated tissue to achieve chemically and orientationally sensitive high-resolution images.
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