Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Partial characterization of a mitomycin-C resistant T cell.

R E Anderson, J C Standefer, G M Troup

    Journal of Leukocyte Biology
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Y chromosome polymorphisms in native American and Siberian populations: identification of native American Y chromosome haplotypes.

    Human genetics·1997
    Same author

    Strategy for distinguishing a new DQB1 allele (DQB1*0611) from the closely related DQB1*0602 allele via sequence specific PCR or direct DNA sequencing.

    Tissue antigens·1996
    Same author

    HLA-B alleles of the Navajo: no evidence for rapid evolution in the Nadene.

    Tissue antigens·1996
    Same author

    Polymorphic human specific Alu insertions as markers for human identification.

    Electrophoresis·1995
    Same author

    HLA-DR53 protects against thrombotic thrombocytopenic purpura/adult hemolytic uremic syndrome.

    American journal of hematology·1994
    Same author

    A new DRB1 allele (DRB1*0811) identified in Native Americans.

    Immunogenetics·1994

    This study describes a unique T cell resistant to mitomycin-C, a drug that often inhibits immune responses. This cell

    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Mitomycin-C is commonly used to inactivate immune cells in vitro.
    • Certain lymphocytes exhibit resistance to mitomycin-C, impacting experimental outcomes.
    • Understanding lymphocyte subsets and their drug sensitivities is crucial for immunology research.

    Purpose of the Study:

    • To characterize a specific T cell population resistant to mitomycin-C.
    • To investigate the mechanisms underlying mitomycin-C resistance in lymphocytes.
    • To explore the potential of reductase activity as a marker for lymphocyte subsets.

    Main Methods:

    • Characterization of mitomycin-C resistant cells using functional assays (e.g., concanavalin A stimulation).
    • Assessment of cell adherence properties (nylon wool).

    Related Experiment Videos

  • Analysis of cell distribution in lymphoid tissues.
  • Evaluation of radiosensitivity.
  • Measurement of microsomal reductase activity involved in mitomycin-C activation.

    • Main Results:

    • A T cell population resistant to mitomycin-C was identified.
    • This resistant T cell exhibited responsiveness to concanavalin A, poor adherence to nylon wool, higher prevalence in lymph nodes, and relative radiosensitivity.
    • Reduced microsomal reductase activity was observed in these resistant cells, correlating with their resistance to mitomycin-C.
    • Variations in reductase activity suggest potential for differentiating lymphocyte subsets.

    Conclusions:

    • A distinct T cell subset demonstrates intrinsic resistance to mitomycin-C.
    • Lower microsomal reductase activity is a key factor in this mitomycin-C resistance.
    • Microsomal reductase activity can serve as a marker to distinguish between lymphocyte subsets.