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Updated: Jan 26, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A resorufin-based fluorescent probe for imaging polysulfides in living cells
1Center of Basic Molecular Science, Department of Chemistry, Tsinghua University, Beijing, 10084, China. yinzheng@mail.tsinghua.edu.cn.
Abstract:
As the oxidative metabolites of the endogenous gasotransmitter H2S, H2Sn have attracted ever-increasing attention in the field of biomedical research due to their vital functions in biological systems. Herein, we report a resorufin-based "turn-on" probe for H2Sn sensing. An SNAr substitution-intramolecular cyclization cascade reaction was used in this probe for detecting exogenous and endogenous H2Sn. The detection process could be monitored using UV-Vis and fluorescence spectroscopy and the naked eye. The emission response of the probe towards H2Sn presented a good linear relationship in the 0-50 μM concentration range, and the LOD of this probe was 24 nM. The probe was used successfully to visualize endogenous H2Sn generated in the RAW 246.7 cells under external stimulation.
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