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Updated: Jan 26, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Fabrication of silver nanoclusters with enhanced fluorescence triggered by ethanol solvent: a selective fluorescent
Shu Huan Ren1, Shi Gang Liu1, Yu Ling1
1Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Abstract:
We present a facile method for the preparation of red-emitting and water-soluble silver nanoclusters (Ag NCs) using dihydrolipoic acid and sodium borohydride as the template and reducing agent. Ethanol solvent is demonstrated to endow Ag NCs with dramatically enhanced fluorescence; therefore, the Ag NCs are synthesized in ethanol/water solution (e/w-Ag NCs) instead of aqueous solution. Specific trivalent chromium (Cr3+) recognition capability of the e/w-Ag NCs can thus be obtained on the basis of its fluorescence quenching. The mechanisms for fluorescence enhancement and quenching of the e/w-Ag NCs triggered by ethanol and Cr3+, respectively, are investigated in detail. Next, a fluorescence method for detection of Cr3+ is established and its analytical performance is evaluated: the detection limit for Cr3+ is 0.71 μM and the linear range is from 2 to 40 μM. The fluorescent probe exhibits sufficient sensitivity and good selectivity toward Cr3+, illustrating that it has great promise for practical application in Cr3+ detection.
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