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Updated: Jan 26, 2026

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes
Published on: December 22, 2013
Identification and Expression Analysis of Chitinase Genes in Pitchers of Nepenthes sp. during Development
M A Filyushin1, E Z Kochieva2,3, A V Shchennikova2
1Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia. michel7753@mail.ru.
Abstract:
Fifteen chitinases of classes I-V were identified in the transcriptomes of pitchers and adult leaves of the carnivorous plant Nepenthes sp. Ten of these chitinases were identified for the first time, including the chitinases of classes II and V. The expression levels of all found chitinase genes in leaves and at three stages of pitcher development were determined. The maximum level of transcriptional activity in an open pitcher was observed for the genes encoding chitinase NChi4 (class II) and its isoforms. The expression levels of these genes significantly increased as the pitcher developed. In addition, for the first time, transcription of the genes encoding chitinases of all five classes was detected in the leaves of this plant.
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