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Modification and Clinical Application of the Inner Perivitelline Membrane Test in Different Avian Species

Judith Krohn1, Dominik Fischer2, Helena Schneider3

  • 1Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals with Ambulatory Service, Faculty of Veterinary Medicine, Justus-Liebig-University, Frankfurter Str. 106, 35392 Giessen, Germany. judith.krohn@vetmed.uni-giessen.de.

Veterinary Sciences
|April 25, 2019
PubMed

Insights

This study adapted the inner perivitelline membrane (IPVM) test for avian sperm penetration. Chicken IPVM is suitable for rooster sperm quality assessment but not for interspecies assays in other birds.

Area of Science:

  • Reproductive Biology
  • Avian Science
  • Spermatozoa Function Testing

Background:

  • Assessing avian spermatozoa penetration ability is crucial for reproductive efficiency.
  • The inner perivitelline membrane (IPVM) offers a potential substrate for sperm penetration assays.
  • Developing reliable interspecies assays remains a challenge in avian reproduction.

Purpose of the Study:

  • To adapt the inner perivitelline membrane (IPVM) test as an interspecies penetration assay for avian spermatozoa.
  • To evaluate the IPVM's suitability for testing sperm penetration across different avian species.
  • To optimize the IPVM isolation protocol for practical application.

Main Methods:

  • Isolation of IPVM from various avian species using modified acid hydrolysis.
  • Evaluation of IPVM integrity and hole formation using dark field microscopy.
  • Testing the penetrating ability of rooster, cockatiel, and falcon spermatozoa on chicken IPVM.

Main Results:

  • Egg incubation time significantly impacted IPVM isolation success (p < 0.0001).
  • Successful IPVM isolation was limited to two days post-incubation.
  • Chicken IPVM showed 100% penetration by rooster sperm (fresh and frozen-thawed) and 10% by cockatiel sperm; falcon sperm failed.

Conclusions:

  • Chicken IPVM is effective for evaluating cryopreserved rooster sperm quality.
  • Chicken IPVM is unsuitable for establishing a functional interspecies sperm penetration assay for tested avian species.
  • Further research is needed to develop reliable interspecies avian sperm penetration assays.
Keywords:
assisted reproductionbirdfunctional sperm-assayperivitelline-membrane-penetration

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