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Updated: Jan 25, 2026

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Ratiometric Imaging of Cysteine Level Changes in Endoplasmic Reticulum during H2O2-Induced Redox Imbalance
Baoli Dong1, Yaru Lu1, Nan Zhang1
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering , University of Jinan , Jinan , Shandong 250022 , People's Republic of China.
Abstract:
Cysteine (Cys) is an important mediator to regulate the redox state of endoplasmic reticulum (ER), and its level is closely related with many ER stress induced serious diseases. Herein, we present an ER-specific fluorescent probe for the ratiometric imaging of cellular Cys for the first time. The probe exhibited desirable selectivity and sensitivity to Cys. Biological imaging experiments demonstrated that the probe possessed an ER-targeting property, showed ratiometric response to Cys in ER, and could be applied for the ratiometric imaging of Cys level changes during H2O2-induced redox imbalance in living cells.
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