Related Experiment Video
Updated: Jan 25, 2026

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
Published on: June 19, 2019
The prognostic significance of bone marrow involvement in diffuse large B cell lymphoma according to the flow
Uri Greenbaum1,2, Itai Levi1,2, Odelia Madmoni1,2
1Department of Hematology, Soroka University Medical Center , Beer Sheba , Israel.
Abstract:
The significance of minimal bone marrow (BM) involvement in diffuse large B cell lymphoma (DLBCL), as determined by flow cytometry (FC), is unclear. Patient outcomes were retrospectively analyzed based on their BM biopsy and FC involvement. Eighty-one patients were included, 21 and 51 were positive for biopsy(B+) and FC(FC+) respectively. B+ FC+ patients had a 52.3%CR rate, the B- FC+ group had 76.7%, and the B- FC- had 73.3%. Mean time to progression (TTP) was 67.45, 76.8, and 79.3 months and median Overall survival(OS) was 54.4, 76.6 and 69.5 months for the B+ FC+, B- FC+, and B- FC- groups respectively. A cutoff of 1% pathologic cells was an independent risk factor for TTP. In a multivariable analysis including International Prognostic Index (IPI), sex and HB, FC+ was independently associated with TTP (but not OS) at 5 years (HR 2.64, 95%CI 1.03-6.77) and at 7 years(HR 2.83, 95%CI 1.08-7.39). In summary, FC determined minimal involvement may suggest an intermediate risk group of DLBCL patients.
Related Concept Videos
Flow Cytometry
In...
Bone Marrow Sampling and Transplants
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
Diffusion
Statistical Significance
Cholesterol: Significance and Regulation
Considering cholesterol and...
The Significance of Membrane Transport
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...

