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Published on: March 31, 2008
Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Ashwathama Dilipkumar1,2, Alaa Al-Shemmary1,2, Lucas Kreiß1,2
1Institute of Medical Biotechnology Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) Paul-Gordan-Str. 3 91052 Erlangen Germany.
Abstract:
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side-viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.
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