Related Experiment Video
Updated: Jan 25, 2026
04:38
Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
Published on: March 28, 2018
16.5K
Ex Vivo Model in Cholestasis Research
Ruby E H Karsten1, Dorenda Oosterhuis2, Louise A van Wijk2
1Department of Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|April 25, 2019
Summary
Developing a new in vitro model is crucial for studying cholestasis. Precision-cut liver slices offer a physiologically relevant system to understand drug-induced liver injury and bile flow.
Area of Science:
- Hepatology
- Toxicology
- In vitro modeling
Background:
- Mimicking human cholestasis in vitro requires complex multicellular systems that current models fail to provide.
- There is a significant need for in vitro models that preserve cell-cell interactions and functional bile flow.
Purpose of the Study:
- To describe the preparation and culturing of human precision-cut liver slices (PCLS).
- To present a protocol for utilizing PCLS to predict drug-induced cholestatic liver injury.
Main Methods:
- Precision-cut liver slices (PCLS) preparation and long-term culturing.
- Application of PCLS for assessing drug-induced cholestasis.
Main Results:
- PCLS replicate key multicellular characteristics of the human liver.
- The described protocol enables the prediction of drug-induced cholestatic liver injury.
Conclusions:
- Precision-cut liver slices (PCLS) provide a valuable ex vivo model for studying liver physiology and disease.
- This technique advances the prediction of drug-induced liver injury, particularly cholestasis.
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