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C2 and factor B: structure and genetics.

D R Bentley, R D Campbell

    Biochemical Society Symposium
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Complement components C2 and factor B are serine proteases within the major histocompatibility complex. DNA analysis reveals greater variability at the C2 locus than previously detected by protein typing.

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    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Biochemistry

    Background:

    • Complement components C2 and factor B are serine proteases encoded by loci in the human major histocompatibility complex on chromosome 6.
    • These proteins share significant homology, particularly in their catalytic and non-catalytic domains.

    Purpose of the Study:

    • To investigate the genetic structure and polymorphism of complement components C2 and factor B.
    • To compare the variability detected at the DNA level with protein-level typing.

    Main Methods:

    • Molecular mapping and DNA sequence analysis of the C2 and factor B genes.
    • Analysis of protein variants by charge differences.
    • Restriction fragment length polymorphism (RFLP) analysis of the C2 gene.

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    Main Results:

    • The factor B gene is 6 kb with 18 exons; the C2 gene is 18 kb, with a 425 bp intergenic region.
    • Both C2 and factor B exhibit polymorphism, with two common factor B alleles (F and S) identified.
    • DNA polymorphisms subdivide the C2 allotype C2C, indicating greater C2 locus variability than previously observed.

    Conclusions:

    • The C2 and factor B genes possess distinct structural features and are located closely on chromosome 6.
    • DNA-level analysis reveals a higher degree of polymorphism at the C2 locus than protein typing suggests.
    • These findings enhance our understanding of complement system genetic diversity within the MHC.