Related Experiment Video

Updated: Jan 25, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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Lizard Tail Extracts Exert Protective Effect Against Oxidative Stress-Induced Cellular Senescence in Human

Joo Eon Lee1,2, Mira Kim1, Soon Yong Park1

  • 11 Department of Biological Science, Dong-A University, Busan, Korea.

Journal of Medicinal Food
|April 25, 2019
PubMed

Insights

Lizard tail extracts (LTE) show anti-aging effects on human fibroblasts by reducing oxidative stress and preventing cellular senescence. This research highlights LTE

Area of Science:

  • Biogerontology
  • Dermatology
  • Molecular Biology

Background:

  • Lizard tail extracts (LTE) are known to inhibit cancer cell proliferation and angiogenesis without toxicity to human fibroblasts.
  • Limited research exists on the application of LTE for skin diseases, particularly oxidative stress-induced aging.

Purpose of the Study:

  • To investigate the anti-aging effects of LTE on human fibroblasts.
  • To determine LTE's impact on cellular senescence and oxidative stress in skin cells.

Main Methods:

  • SA-β-galactosidase staining to assess cellular senescence.
  • 2',7'-dichlorofluorescein diacetate staining to measure reactive oxygen species (ROS) levels.
  • Western blot analysis to examine underlying signaling pathways.

Main Results:

  • LTE demonstrated a significant anti-aging effect on human fibroblasts, confirmed by reduced SA-β-galactosidase activity.
  • LTE treatment decreased hydrogen peroxide-induced ROS levels in fibroblasts.
  • Western blot analysis indicated that LTE suppresses ROS and downregulates p21 expression.

Conclusions:

  • Lizard tail extracts possess anti-aging properties beneficial for human skin.
  • LTE prevents cellular senescence by mitigating oxidative stress and downregulating the p21 pathway.

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