Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A High-Throughput Microtiter-Based Fungicide Sensitivity Assay for Oomycetes Using Z'-Factor Statistic.

Zachary A Noel1,2, Alejandro J Rojas1,2, Janette L Jacobs1

  • 1Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824.

Phytopathology
|April 25, 2019
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global population structures and demographic history of Suillus luteus, a pine co-introduced ectomycorrhizal fungus associated with exotic forestry and invasion.

The New phytologist·2026
Same author

Fungicide Effects on Mycotoxins and Forage Quality on a Brown Midrib and a Dual-Purpose Silage Corn Hybrid.

Plant disease·2026
Same author

Genome sequence of the dimorphic <i>Dothioraceae</i> fungal isolate EMM_F3.

Microbiology resource announcements·2026
Same author

Modeling the environment-related risk of frogeye leaf spot (Cercospora sojina) in soybean across the United States.

Scientific reports·2026
Same author

Current Understanding of the Genetic and Molecular Interactions Between the Tar Spot Pathogen <i>Phyllachora maydis</i> and Maize.

Molecular plant-microbe interactions : MPMI·2026
Same author

Global population structures and demographic history of <i>Suillus luteus</i> , a pine co-introduced ectomycorrhizal fungus associated with exotic forestry and invasion.

bioRxiv : the preprint server for biology·2026

A new high-throughput assay quantifies oomycete fungicide sensitivity faster than traditional methods. This tool improves fungicide resistance monitoring for oomycete populations and communities.

Area of Science:

  • Plant Pathology
  • Mycology
  • Agricultural Science

Background:

  • Assessing oomycete fungicide sensitivity is crucial but current methods are slow and labor-intensive.
  • Microtiter-based assays offer higher throughput but require robust quality control for reproducibility.
  • Developing efficient and reliable methods for assay quality detection is essential for accurate fungicide phenotyping.

Purpose of the Study:

  • To develop and validate a robust, high-throughput fungicide phenotyping assay for oomycetes.
  • To implement quality control measures, including the Z' factor and growth curves, for assay reliability.
  • To compare the accuracy and precision of the new assay against the established amended medium (gold standard) method.

Main Methods:

  • Spectrophotometric quantification of oomycete mycelial growth in liquid culture.
Keywords:
disease control and pest managementtechniques

Related Experiment Videos

  • Utilized Z' factor for quality control, ensuring sufficient growth in control conditions.
  • Employed growth curves to confirm active growth during EC50 estimation.

    • Main Results:

    • The high-throughput assay demonstrated accuracy comparable to the gold standard method.
    • Concordance correlation indicated high accuracy, though precision may be slightly lower than the amended medium assay.
    • Successfully tested the sensitivity of 216 oomycete isolates to mefenoxam and ethaboxam.

    Conclusions:

    • The developed assay enables efficient, high-throughput fungicide phenotyping of oomycetes.
    • This method facilitates fungicide sensitivity assessment at population and community levels.
    • The assay's quality control mechanisms ensure reliable data for fungicide resistance monitoring.