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miR-144 reverses cisplatin resistance in cervical cancer via targeting LHX2

Fan Shi1, Jin Su1, Zi Liu1

  • 1Department of Radiation Oncology, First Hospital of Xi'an Jiaotong Univesity, Xi'an, Shaanxi, China.

Journal of Cellular Biochemistry
|April 25, 2019
PubMed

Insights

MicroRNA-144 (miR-144) overcomes cisplatin resistance in cervical cancer by targeting LIM homeobox 2 (LHX2). This enhances apoptosis and reduces invasion, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs play crucial roles in cancer development and chemoresistance.
  • Cervical cancer often exhibits resistance to chemotherapy agents like cisplatin (CDDP).
  • Understanding the mechanisms of chemoresistance is vital for improving treatment outcomes.

Purpose of the Study:

  • To investigate the role and mechanism of microRNA-144 (miR-144) in cisplatin resistance in cervical cancer cells.
  • To determine if miR-144 targets LIM homeobox 2 (LHX2) and influences chemoresistance.
  • To evaluate the therapeutic potential of modulating miR-144 in cervical cancer.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were used to measure miR-144 and LHX2 expression.
  • Functional assays assessed the impact of miR-144 overexpression on cell viability, apoptosis, and invasion.
  • A luciferase reporter assay confirmed the direct binding of miR-144 to the 3'-UTR of LHX2 mRNA.

Main Results:

  • Overexpression of miR-144 reduced cell viability, induced apoptosis, and inhibited migration and invasion in cisplatin-treated cervical cancer cells.
  • miR-144 directly targets the 3'-untranslated region (3'-UTR) of LIM homeobox 2 (LHX2) mRNA, decreasing its expression.
  • Restoration of LHX2 partially reversed the effects of miR-144, indicating its critical role.

Conclusions:

  • miR-144 overcomes cisplatin resistance in cervical cancer by targeting and downregulating LHX2.
  • This mechanism involves promoting apoptosis and inhibiting invasion.
  • miR-144 represents a potential therapeutic target for overcoming chemoresistance in cervical cancer.
Keywords:
cervical cancercisplatinmiR-144resistance LXH2

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