Related Experiment Video

Updated: Jan 25, 2026

Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis
04:22

Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis

Published on: March 3, 2023

851

Fisetin and 5-fluorouracil: Effective combination for PIK3CA-mutant colorectal cancer

Naghma Khan1,2, Farah Jajeh1, Emily L Eberhardt3

  • 1Department of Dermatology, University of Wisconsin-Madison, Madison, WI.

International Journal of Cancer
|April 25, 2019
PubMed

Insights

Fisetin, a dietary flavonoid, shows promise in preventing and treating colorectal cancer. It reduces tumor incidence and enhances 5-fluorouracil (5-FU) therapy efficacy, particularly in PIK3CA-mutant cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Prevention

Background:

  • Colorectal cancer (CRC) arises from genetic alterations, notably PIK3CA mutations.
  • Current treatments like 5-fluorouracil (5-FU) have limited efficacy and significant side effects.
  • Dietary compounds offer potential for cancer prevention and adjuvant therapy.

Purpose of the Study:

  • To investigate the effects of fisetin, alone or with 5-FU, on colorectal cancer cell growth and tumorigenesis.
  • To explore the impact of fisetin and 5-FU on the PI3K/AKT/mTOR signaling pathway.
  • To evaluate fisetin as a preventive agent and adjuvant therapy for PIK3CA-mutant CRC.

Main Methods:

  • In vitro studies assessed cell viability, colony formation, and apoptosis in PIK3CA-mutant and wild-type colon cancer cells.
  • Western blotting analyzed protein expression and phosphorylation in the PI3K/AKT/mTOR and AMPK signaling pathways.
  • In vivo studies utilized ApcMin/+ mice with PI3K activation to evaluate fisetin and 5-FU effects on intestinal tumorigenesis.

Main Results:

  • Fisetin and 5-FU combination therapy downregulated PI3K/AKT/mTOR signaling and increased AMPKα phosphorylation in PIK3CA-mutant cells.
  • Fisetin significantly reduced tumor incidence in ApcMin/+ mice with PI3K activation.
  • Combined fisetin and 5-FU treatment decreased the overall number of intestinal tumors in the mouse model.

Conclusions:

  • Fisetin demonstrates strong potential as a preventive agent against intestinal tumorigenesis.
  • Fisetin can serve as an effective adjuvant therapy when combined with 5-FU for PIK3CA-mutant colorectal cancer.
  • This study highlights fisetin's therapeutic value in CRC, offering a novel approach to improve treatment outcomes.
Keywords:
PIK3CA5-fluorouracilPI3K/AKT/mTOR signalingcolorectal cancer

More Related Videos

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer06:49

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Published on: February 10, 2023

2.7K
Orthotopic Mouse Model of Colorectal Cancer08:43

Orthotopic Mouse Model of Colorectal Cancer

Published on: December 4, 2007

47.1K

Related Concept Videos

Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
6.8K
Combined Effects of Drugs: Antagonism01:30

Combined Effects of Drugs: Antagonism

The combined effects of drugs can result in various interactions, of which an important type is antagonism. Antagonism is a mechanism where one drug inhibits or counteracts the effects of another drug. Antagonism can occur through various means, including receptor binding, allosteric modulation, functional interaction, chemical reactions, and pharmacokinetic processes.
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
11.6K
5-Number Summary01:04

5-Number Summary

In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot.
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
5.5K
Framing Effects03:26

Framing Effects

Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
7.9K
Buffer Effectiveness02:19

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.0K
Biological Effects of Radiation02:59

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
17.7K