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Updated: Jan 25, 2026

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

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Quantitative Comparison of Selected Virulence Associated Characteristics in Food and Clinical Isolates of Listeria

Fernando Del Corral1, Robert L Buchanan1, Maryanne M Bencivengo1

  • 1Microbial Food Safety Research Unit, U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 East Mermaid Lane, Philadelphia, Pennsylvania 19118.

Journal of Food Protection
|April 26, 2019
PubMed

Insights

Listeria monocytogenes isolates were lethal to mice, hemolytic, and invasive, unlike Listeria ivanovii. Virulence factors varied, suggesting additional factors influence Listeria pathogenicity.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Cell Biology

Background:

  • Listeria species are important foodborne pathogens.
  • Understanding virulence factors is crucial for assessing pathogenicity.

Purpose of the Study:

  • To quantitatively compare virulence markers of Listeria monocytogenes and Listeria ivanovii.
  • To investigate potential differences between food and clinical isolates.
  • To explore the relationship between different virulence factors.

Main Methods:

  • Quantitative comparison of lethality in immunocompromised mice.
  • Assays for hemolytic activity, epithelial cell invasiveness, and cytotoxicity.
  • Light and electron microscopy of infected cells.

Main Results:

  • All Listeria monocytogenes isolates were hemolytic, invasive, weakly cytotoxic, and lethal to mice.
  • Listeria ivanovii isolates showed similar properties but were non-virulent.
  • Little quantitative correlation was found among virulence markers.
  • No systematic differences were observed between food and clinical isolates.
  • Both species were observed within an actin matrix in infected cells.

Conclusions:

  • Listeria monocytogenes exhibits greater virulence than Listeria ivanovii in this model.
  • Virulence factor correlation suggests additional pathogenicity factors for L. monocytogenes.
  • Food and clinical isolates did not show distinct virulence profiles.
  • Actin-mediated internalization is a common feature for both species.

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