Related Experiment Video
Updated: Jan 25, 2026
07:15
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
881
Mesenchymal stem cells targeting PI3K/AKT pathway in leukemic model
Esraa S A Ahmed1, Neamat H Ahmed1, Amina M Medhat2
11 National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
Summary
Mesenchymal stem cells (MSCs) show promise in treating leukemogenesis by improving blood cell production and reducing inflammation. MSCs modulate key signaling pathways like PI3K/AKT, offering a potential alternative to growth factors in cancer therapy.
Area of Science:
- Hematology
- Oncology
- Stem Cell Biology
Background:
- Leukemogenesis involves dysregulated cellular signaling and inflammation.
- Mesenchymal stem cells (MSCs) possess immunomodulatory and anti-inflammatory properties.
- The role of MSCs in modulating signaling pathways in leukemogenesis is not fully understood.
Purpose of the Study:
- To investigate MSCs as an alternative to cellular signaling growth factors in rat leukemogenesis.
- To analyze the impact of MSCs on PI3K/AKT/mTOR signaling pathway in leukemogenesis.
- To evaluate MSCs' therapeutic potential in improving hematopoiesis and reducing inflammation.
Main Methods:
- Induction of leukemogenesis in rats using 7,12-dimethyl benz [a] anthracene (DMBA).
- Assessment of hematological parameters, bone marrow oxidative and inflammatory indices (TGF-β, IL-6).
- Western immunoblotting to analyze PI3K, AKT, mTOR, and PTEN protein levels.
- Gene expression analysis for BCL-2, CXCR4, and BAX.
- Evaluation of MSCs' therapeutic effects on hematopoiesis, inflammation, apoptosis, and bone marrow homing.
Main Results:
- DMBA treatment significantly decreased hematological parameters and increased bone marrow inflammation (TGF-β, IL-6).
- DMBA-induced leukemogenesis showed increased PI3K, AKT, mTOR, and BCL-2, with decreased PTEN, CXCR4, and BAX.
- MSC treatment significantly improved hematological parameters and reduced inflammatory markers.
- MSCs modulated the PI3K/AKT signaling pathway, induced apoptosis, and enhanced bone marrow homing.
Conclusions:
- MSCs effectively improve hematopoiesis and alleviate inflammation in experimental leukemogenesis.
- MSC therapy modulates the PI3K/AKT signaling pathway, offering a therapeutic strategy for leukemogenesis.
- MSCs represent a promising alternative for cellular signaling growth factors in managing leukemogenesis.
Keywords:
B cell lymphoma-2 proteinB cell lymphoma-2 protein–associated x-proteinC-X-C motif chemokine receptor-4LeukemogenesisPI3Kmesenchymal stem cellsMore Related Videos
Related Concept Videos
01:22PI3K/mTOR/AKT Signaling Pathway
5.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.4K
01:19Mesenchymal Stem Cells
5.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.5K
01:33Adult Stem Cells
33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K
00:58Embryonic Stem Cells
32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K
01:27C4 Pathway and CAM
48.9K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
48.9K
01:13Induced Pluripotent Stem Cells
27.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K