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The Lactococcus lactis Pan-Plasmidome.

Philip Kelleher^1,2, Jennifer Mahony^1,2, Francesca Bottacini²

¹School of Microbiology, University College Cork, Cork, Ireland.

Frontiers in Microbiology

|April 26, 2019

Summary

This summary is machine-generated.

Researchers identified 83 plasmids in Lactococcus lactis strains, including megaplasmids. A combined sequencing approach improves detection of these extrachromosomal genetic elements and their traits.

Keywords:

SMRT sequencing conjugation dairy fermentation lactic acid bacteria lactococcal plasmid

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Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Plasmids are extrachromosomal genetic elements crucial for bacterial adaptation and survival.
Understanding the plasmid content of bacterial strains like Lactococcus lactis is vital for biotechnological applications.

Purpose of the Study:

To characterize the plasmidome of Lactococcus lactis strains.
To evaluate sequencing technologies for comprehensive plasmid detection.
To investigate biotechnologically relevant plasmid-encoded traits.

Main Methods:

Genome sequencing of 16 Lactococcus lactis strains using Pacific Biosciences SMRT sequencing.
Comparative analysis of plasmid sequences with publicly available data.
Combined Illumina/SMRT sequencing approach proposed for improved detection.

Main Results:

Identification of 83 plasmids, including two megaplasmids, across 16 Lactococcus lactis strains.
Assessment of limitations in SMRT sequencing for complete plasmid profiling.
Definition of the lactococcal plasmidome and identification of key traits.

Conclusions:

A combined sequencing strategy enhances the detection of the full plasmid complement.
The lactococcal plasmidome harbors traits important for conjugation, bacteriocin, EPS, and phage resistance.
This study provides a foundation for understanding plasmid diversity and function in Lactococcus lactis.