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Updated: Jan 25, 2026

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
Published on: May 14, 2016
Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro
Lucía Paniagua-Herranz1,2,3, Rosa Gómez-Villafuertes1,2,3, David de Agustín-Durán1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary Medicine, Complutense University, Madrid, Spain.
Abstract:
A comprehensive understanding of the mechanisms controlling the behavior of cell populations with regenerative potential is the first step to design effective therapeutic strategies for many diseases. However, a precise description of the biological events involved, such as proliferation, differentiation, cell fate decisions, migration, or viability, may be hampered by the classical use of experiments based on end-point analysis. By contrast, live imaging and single cell tracking provides researchers with an accurate readout of these features in cells throughout an experiment. Here, we describe a protocol to apply time-lapse video microscopy and post-processing of the data to study critical aspects of the biology and the lineage progression of multiple neural populations.
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