Reduced insulin sensitivity in young, normoglycaemic subjects alters microvascular tissue oxygenation during...

What is the central question of the study? Are measures of reduced insulin sensitivity in young, normoglycaemic subjects correlated with near-infrared spectroscopy-derived microvascular responsiveness [tissue oxygen saturation reperfusion rate (STO₂ upslope)] during postocclusive reactive hyperaemia? What is the main finding and its importance? A sevenfold range of hepatic insulin sensitivity is significantly correlated (r = 0.44, P = 0.02) with STO₂ upslope after transient tissue ischaemia. Near-infrared spectroscopy may be an important tool for determining altered microvascular function before onset of hyperglycaemia. Identification of pre-type 2 diabetes much earlier than with the present clinical criteria is important for pre-emptive measures against microvascular deterioration.

Near-infrared spectroscopy (NIRS) measurement of postocclusive reactive hyperaemia (PORH) tissue oxygen saturation reperfusion rate [STO₂ upslope (as a percentage per minute)] has recently been correlated with the percentage of flow-mediated dilatation (%FMD). Cardiovascular disease is associated with impairments in %FMD. Reduced insulin sensitivity may negatively affect the vascular system for many years before prediabetes/type 2 diabetes states. The aim of this study was to determine whether static and dynamic STO₂ parameters during PORH are correlated with reduced insulin sensitivity in young, normoglycaemic subjects. Glucose and insulin were measured during an oral glucose tolerance test in 18- to 26-year-old, healthy subjects (11 men and 11 women), and STO₂ was measured during PORH of antebrachial muscle. Hepatic (ISI_HOMA ) and whole-body (ISI_COMP ) insulin sensitivities were calculated. The STO₂ upslope was negatively correlated with minimal STO₂ (r = -0.5, P = 0.01). The change of STO₂ from minimum to baseline (ΔSTO₂ ) was significantly negatively correlated with fasting insulin (r = -0.5, P = 0.01) and a positively correlated with ISI_HOMA (r = 0.65, P = 0.001). The minimum STO₂ was significantly negatively correlated with ISI_HOMA , and STO₂ upslope was significantly positively correlated with ISI_HOMA (r = 0.44, P = 0.02). The minimum STO₂ (a measure of O₂ extraction while the cuff was inflated), ΔSTO₂ (a measure of the amount of reperfusion) and STO₂ upslope (a measure of responsiveness of the microcirculation to ischaemia) were all positively correlated with ISI_HOMA , one of the longest-used measures of insulin sensitivity. The NIRS-derived STO₂ might be a useful tool for assessing how levels of reduced insulin sensitivity in young, normoglycaemic adults affect the microvasculature.