Related Experiment Video

Updated: Jan 25, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.2K

Circular RNA expression profile in gingival tissues identifies circ_0062491 and circ_0095812 as potential treatment

Jie Li1, Ruiyue Xie1

  • 1Department of Stomatology, Shandong Provincial Qianfoshan Hospital, Jinan, China.

Journal of Cellular Biochemistry
|April 26, 2019
PubMed

Insights

Circular RNAs (circRNAs) are newly found to play a role in periodontal inflammation. Researchers identified key circRNAs, including circ_0062491 and circ_0095812, involved in periodontitis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Immunology

Background:

  • Circular RNAs (circRNAs) are novel noncoding RNAs involved in immune responses.
  • The role of circRNAs in periodontal inflammation remains largely unknown.
  • Periodontitis is a common inflammatory disease affecting gums and supporting bone.

Purpose of the Study:

  • To identify specific circRNAs involved in periodontal inflammation.
  • To explore the function and interactions of dysregulated circRNAs in periodontitis.
  • To validate the expression of key circRNAs in patient samples.

Main Methods:

  • Illumina sequencing of gingival tissues from periodontitis patients and healthy controls.
  • Gene Ontology (GO) enrichment analysis for functional insights.
  • Construction of circRNA-miRNA interaction networks.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) for validation.

Main Results:

  • 1304 dysregulated circRNAs were identified in inflamed gingival tissues.
  • Key circRNAs (circ_0095812, circ_0120299, circ_0125699, circ_0062491, circ_0043115) were highlighted in periodontitis pathobiology.
  • circ_0062491 (downregulated) and circ_0095812 (upregulated) expression patterns were confirmed.
  • circ_0062491 was identified as a sponge for miR-584, a key player in periodontitis.

Conclusions:

  • Specific circRNAs are dysregulated in periodontal inflammation.
  • circ_0062491 and circ_0095812 are potential biomarkers for periodontitis.
  • The circ_0062491/miR-584 axis may be a critical pathway in periodontitis pathogenesis.
Keywords:
circ_0062491circ_0095812circular RNAsmicroRNAsperiodontitis

More Related Videos

Identification of Circular RNAs using RNA Sequencing08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.7K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.4K

Related Concept Videos

Deformation in a Circular Shaft01:10

Deformation in a Circular Shaft

One of the distinctive characteristics of circular shafts is their ability to maintain their cross-sectional integrity under torsion. In other words, each cross-section continues to exist as a flat, unaltered entity, simply rotating like a solid, rigid slab. To understand the distribution of shearing stress within such a shaft, consider a cylindrical section inside this circular shaft. This section has a length of L and a radius of R, with one end fixed. The radius of the cylindrical section is...
882
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.9K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.5K
Uniform Circular Motion01:14

Uniform Circular Motion

Uniform circular motion is a specific type of motion in which an object travels in a circle with a constant speed. For example, any point on a propeller spinning at a constant rate is undergoing uniform circular motion. The second, minute, and hour hands of a watch also undergo uniform circular motion. It is hard to believe that points on these rotating objects are actually accelerating, even though the rotation rate is constant. To understand this, we must analyze the motion in terms of...
21.6K
Non-uniform Circular Motion01:22

Non-uniform Circular Motion

In uniform circular motion, the particle executing circular motion has a constant speed, and the circle is at a fixed radius. However, not all circular motion occurs at a constant speed. A particle can travel in a circle and speed up or slow down, showing an acceleration in the direction of motion. In that case, the motion is called non-uniform circular motion, and an additional acceleration is introduced, which is in the direction tangential to the circle. 
For example, such...
9.5K
Dynamics of Circular Motion01:30

Dynamics of Circular Motion

An object undergoing circular motion, like a race car, is accelerating because it is changing the direction of its velocity. This centrally directed acceleration is called centripetal acceleration. This acceleration acts along the radius of the curved path (thus is also referred to as radial acceleration).
Any acceleration must be produced by some force. Therefore, any force or combination of forces can cause centripetal acceleration. A few examples include the tension in the rope on a...
23.3K