Related Experiment Video
Updated: Jan 25, 2026
09:34
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
Published on: March 18, 2020
8.1K
Kenalactams A-E, Polyene Macrolactams Isolated from Nocardiopsis CG3
Omar Messaoudi1,2,3, Enge Sudarman4,5, Mourad Bendahou2
1Microbial Strain Collection , Helmholtz Centre for Infection Research GmbH (HZI) , Inhoffenstrasse 7 , 38124 Braunschweig , Germany.
Journal of Natural Products
|April 26, 2019
Summary
Researchers discovered five new polyene macrolactams, kenalactams A-E, from Nocardiopsis CG3. Kenalactam E shows cytotoxicity against prostate cancer cells, indicating potential therapeutic applications.
Area of Science:
- Natural Product Chemistry
- Microbiology
- Pharmacology
Background:
- Screening programs aim to identify novel biologically active secondary metabolites.
- Saltpan environments are rich sources of unique microbial strains with potential for producing diverse compounds.
Purpose of the Study:
- To isolate and characterize new polyene macrolactams from a novel Nocardiopsis strain.
- To investigate the biosynthetic origin of these compounds.
- To evaluate the cytotoxic activity of the isolated compounds.
Main Methods:
- Isolation and cultivation of Nocardiopsis CG3 from a saltpan.
- Structure elucidation using Nuclear Magnetic Resonance (NMR) and High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS).
- Determination of absolute configuration via chemical derivatization (Mosher's method).
- Biosynthetic feeding experiments.
- Cytotoxicity assays against human prostate cancer PC-3 cells.
Main Results:
- Five new polyene macrolactams, kenalactams A-E (1-5), were identified.
- The structures and absolute configurations of kenalactams A-E were determined.
- Alanine was identified as the starter unit in the biosynthesis of kenalactam A.
- Kenalactam E exhibited significant cytotoxicity against PC-3 cells (IC50 = 2.1 μM).
Conclusions:
- Nocardiopsis CG3 produces a novel class of polyene macrolactams.
- The study provides insights into the biosynthesis of kenalactam A.
- Kenalactam E demonstrates promising anti-cancer activity, warranting further investigation.
Related Concept Videos
01:24DNA Isolation
44.9K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
44.9K
01:34DNA Isolation
199.2K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.2K
01:20Overview Of Cell Separation And Isolation
7.3K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.3K
01:19Cyclic Processes And Isolated Systems
3.4K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.4K
01:24Techniques for Isolation of Pure Cultures
1.9K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
1.9K
01:12Reduced Mass Coordinates: Isolated Two-body Problem
2.4K
In classical mechanics, the two-body problem is one of the fundamental problems describing the motion of two interacting bodies under gravity or any other central force. When considering the motion of two bodies, one of the most important concepts is the reduced mass coordinates, a quantity that allows the two-body problem to be solved like a single-body problem. In these circumstances, it is assumed that a single body with reduced mass revolves around another body fixed in a position with an...
2.4K