Related Experiment Video
Updated: Jan 25, 2026

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
Published on: March 18, 2020
Kenalactams A-E, Polyene Macrolactams Isolated from Nocardiopsis CG3
Omar Messaoudi1,2,3, Enge Sudarman4,5, Mourad Bendahou2
1Microbial Strain Collection , Helmholtz Centre for Infection Research GmbH (HZI) , Inhoffenstrasse 7 , 38124 Braunschweig , Germany.
Abstract:
In our screening program for new biologically active secondary metabolites, a new strain, Nocardiopsis CG3 (DSM 106572), isolated from the saltpan of Kenadsa, was found to produce five new polyene macrolactams, the kenalactams A-E (1-5). Their structures were elucidated by spectral methods (NMR and HRESIMS), and the absolute configuration was derived by chemical derivatization (Mosher's method). Through a feeding experiment, alanine was proven to be the nitrogen-bearing starter unit involved in biosynthesis of the polyketide kenalactam A (1). Kenalactam E (5) was cytotoxic against human prostate cancer PC-3 cells with an IC50 value of 2.1 μM.
Related Concept Videos
DNA Isolation
DNA Isolation
Overview Of Cell Separation And Isolation
Cyclic Processes And Isolated Systems
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
Techniques for Isolation of Pure Cultures
Reduced Mass Coordinates: Isolated Two-body Problem

