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Related Experiment Videos

Nuclear factors binding specific sequences within the immunoglobulin enhancer interact differentially with other

U Schlokat, D Bohmann, H Schöler

    The EMBO Journal
    |December 1, 1986
    PubMed
    Summary

    Cellular factors bind to the mouse immunoglobulin heavy chain (IgH) enhancer, crucial for lymphoid-specific gene expression. Surprisingly, similar factors are used across different cell types, suggesting shared regulatory mechanisms.

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    Area of Science:

    • Molecular Biology
    • Immunology
    • Genetics

    Background:

    • The mouse immunoglobulin heavy chain (IgH) enhancer is vital for lymphoid-specific gene expression.
    • In vivo and in vitro studies suggest cellular factors interact with IgH enhancer sequences.
    • These interactions are inferred to be essential for transcriptional stimulation.

    Purpose of the Study:

    • To investigate the nuclear factors interacting with the IgH enhancer.
    • To compare the binding patterns of these factors in B-cells, T-cells, and HeLa cells.
    • To identify specific DNA-binding factors within the IgH enhancer's central fragment.

    Main Methods:

    • DNase I protection assays.
    • Dimethylsulfate (DMS) protection assays.

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  • Comparison of nuclear extracts from B-cells, T-cells, and HeLa cells.

    • Main Results:

    • Specific interaction of three nuclear factors with the central PstI-EcoRI fragment of the IgH enhancer was demonstrated.
    • Minimal differences were observed in factor binding between B-cell, T-cell, and HeLa cell extracts.
    • Two IgH enhancer-binding factors are shared with polyoma virus and lymphotropic papova virus enhancers.

    Conclusions:

    • The IgH enhancer utilizes specific nuclear factors for its function.
    • These factors appear to be shared across different cell types, including non-lymphoid cells.
    • Shared regulatory factors suggest common mechanisms for transcriptional enhancement in diverse cellular contexts.