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Expression of plasma microRNA-145-5p and its correlation with clinical features in patients with refractory epilepsy

Chun-Hong Shen1, Yin-Xi Zhang1, Yang Zheng1

  • 1Department of Neurology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China.

Epilepsy Research
|April 26, 2019
PubMed
Abstract

Insights

Decreased miRNA-145-5p in plasma may serve as a biomarker for refractory epilepsy. This finding aids in early detection and clinical assessment of epilepsy patients.

Area of Science:

  • Neuroscience
  • Biomarker Discovery
  • Molecular Biology

Background:

  • MicroRNAs (miRNAs) are increasingly recognized for their role in neurological disorders.
  • Epilepsy, a chronic brain condition, presents diagnostic challenges.
  • miRNAs show potential as accessible biomarkers in various brain diseases.

Purpose of the Study:

  • To investigate the expression levels of miRNA-145-5p in patients with refractory epilepsy.
  • To explore the correlation between miRNA-145-5p expression and clinical characteristics of epilepsy.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze plasma miRNA-145-5p levels.
  • A cohort of 40 refractory epilepsy patients and 42 healthy controls was studied.
  • Statistical analysis was performed using IBM SPSS Statistics.

Main Results:

  • Plasma miRNA-145-5p was significantly downregulated in refractory epilepsy and mesial temporal lobe epilepsy (MTLE) patients compared to controls.
  • Receiver Operating Characteristic (ROC) analysis indicated diagnostic potential for refractory epilepsy (AUC=0.632) and MTLE (AUC=0.829).
  • Lower miRNA-145-5p levels correlated with earlier epilepsy onset, increased seizure frequency, and a history of seizures.

Conclusions:

  • Reduced miRNA-145-5p expression is a potential non-invasive biomarker for refractory epilepsy.
  • This biomarker may aid in the early detection and clinical evaluation of epilepsy.
  • Further research is warranted to validate these findings and explore therapeutic implications.
Keywords:
MicroRNA-145-5pPlasmaRefractory epilepsy

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