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Targeting Plant ssDNA Viruses with Engineered Miniature CRISPR-Cas14a
Muhammad Zuhaib Khan1, Sibtain Haider1, Shahid Mansoor1
1National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Trends in Biotechnology
|April 27, 2019
Abstract:
CRISPR-Cas14a is a highly compact protein with great potential to be used as a guided genome editing tool for single-stranded (ss) DNA cleavage. Recently isolated from noncultured archaea, its unrestricted and sequence-independent cleavage makes it an ideal tool for engineering resistance against economically important plant ssDNA viruses.
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