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Fluorescence lifetime-based pH sensing by platinum nanoclusters.

Lihua Jin1, Lulu Shi1, Wenjuan Shi1

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Ultrasmall platinum nanoclusters (Pt NCs) function as a novel pH sensor. This fluorescence-lifetime-based sensor offers reliable and stable pH detection in various environments.

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Area of Science:

  • Nanomaterials Science
  • Analytical Chemistry
  • Biomedical Sensing

Background:

  • Ultrasmall platinum nanoclusters (Pt NCs) are emerging materials with unique optical properties.
  • Developing reliable and sensitive pH sensors is crucial for various scientific and medical applications.
  • Existing pH sensing methods can be limited by stability, reversibility, or susceptibility to environmental interference.

Purpose of the Study:

  • To report the first application of ultrasmall Pt NCs as a fluorescence-lifetime-based pH sensor.
  • To investigate the pH-dependent fluorescence properties of Pt NCs.
  • To evaluate the performance of Pt NCs as a reliable pH sensing platform.

Main Methods:

  • Synthesis of ultrasmall Pt nanoclusters (Pt NCs).
  • Characterization of Pt NCs' fluorescence properties, including intensity and lifetime, across a range of pH values.
  • Testing the sensor's performance in physiologically relevant pH ranges (6.02-7.54).
  • Evaluation of sensor stability, reversibility, and anti-jamming capabilities.
  • Demonstration of pH detection in real water and simulated intracellular samples.

Main Results:

  • Pt NCs exhibit strong pH-dependent fluorescence properties due to surface state changes.
  • Both fluorescence intensity and lifetime show a good linear relationship with pH in the 6.02-7.54 range.
  • The Pt NC sensor demonstrates high anti-jamming capability, excellent stability, and good reversibility.
  • Fluorescence lifetime provides a more reliable readout signal, independent of probe concentration and excitation conditions.

Conclusions:

  • Ultrasmall Pt NCs are effective as a novel fluorescence-lifetime-based pH sensor.
  • The sensor offers high reliability, stability, and reversibility for pH detection.
  • This Pt NC-based system shows promise for practical applications in real-world samples and biological environments.