Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autoxidation of Ethers to Peroxides and Hydroperoxides02:23

Autoxidation of Ethers to Peroxides and Hydroperoxides

9.5K
Ethers represent a class of chemical compounds that become more dangerous with prolonged storage because they tend to form explosive peroxides when standing in the air. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly oxidize to form hydroperoxides and dialkyl peroxides.
9.5K
Regioselectivity of Electrophilic Additions-Peroxide Effect02:35

Regioselectivity of Electrophilic Additions-Peroxide Effect

10.4K
In the presence of organic peroxides, the addition of hydrogen bromide to an alkene yields the isomer that is not predicted by Markovnikov’s rule. For example, the addition of hydrogen bromide to 2-methylpropene in the presence of peroxides gives 1-bromo-2-methylpropane. This addition reaction proceeds via a free radical mechanism, which reverses the regioselectivity. The free radical reaction mechanism involves three stages: initiation, propagation, and termination.
10.4K
Cellular Differentiation00:57

Cellular Differentiation

5.2K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.2K
Cellular Respiration01:18

Cellular Respiration

1.8K
Cellular respiration is a crucial metabolic process through which cells obtain energy from organic substances, mainly glucose, to produce adenosine triphosphate (ATP). This process includes the oxidation of substrates and the transfer of electrons to a separate electron acceptor, facilitating ATP synthesis through a sequence of biochemical reactions.Glycolysis: The Initial StepGlycolysis is the first stage of cellular respiration, occurring in the cytoplasm of both prokaryotic and eukaryotic...
1.8K
Sustainable Development01:43

Sustainable Development

14.8K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
14.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Entrepreneurship beyond the lab: commercializing your creative outputs.

BMC proceedings·2026
Same author

BRD4 recruitment desilences transcription without erasure or depletion of repressive chromatin.

bioRxiv : the preprint server for biology·2026
Same author

Advances in Natural Product Extraction: Established and Emerging Technologies.

Molecules (Basel, Switzerland)·2026
Same author

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources.

Journal of visualized experiments : JoVE·2026
Same author

Puerto Rico Cancer Research Meeting: a venue designed by graduate students to strengthen Puerto Rico's scientific environment.

Molecular biology of the cell·2025
Same author

Direct targeting and regulation of RNA polymerase II by cell signaling kinases.

Science (New York, N.Y.)·2025

Related Experiment Video

Updated: Jan 25, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
13:50

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

12.9K

Development of ergosterol peroxide probes for cellular localisation studies.

Taotao Ling1, Walter H Lang, Michelle M Martinez-Montemayor

  • 1Department of Chemical Biology and Therapeutics, St Jude Children's Research Hospital, Memphis, Tennessee 38105-3678, USA. Fatima.Rivas@stjude.org.

Organic & Biomolecular Chemistry
|April 27, 2019
PubMed
Summary

Ergosterol peroxide shows anticancer potential against triple-negative breast cancer. Chemical probes reveal its distribution in cancer cells, aiding the search for its therapeutic targets.

More Related Videos

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms
08:32

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

1.7K
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.5K

Related Experiment Videos

Last Updated: Jan 25, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
13:50

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

12.9K
Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms
08:32

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

1.7K
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.5K

Area of Science:

  • Medicinal Chemistry
  • Cell Biology
  • Pharmacology

Background:

  • Ergosterol peroxide exhibits broad biological activity, but its mechanism of action is not fully understood.
  • Triple-negative breast cancer (TNBC) remains a challenging disease with limited targeted therapies.

Purpose of the Study:

  • To develop efficient chemical probes for ergosterol peroxide synthesis.
  • To evaluate the in vitro anticancer activity of ergosterol peroxide and its analogues against TNBC models.
  • To investigate the cellular localization and structure-activity relationships of ergosterol peroxide.

Main Methods:

  • Chemical synthesis of ergosterol peroxide analogues.
  • In vitro proliferation assays using TNBC cell lines.
  • Live cell imaging and proteomic profiling to determine cellular distribution.

Main Results:

  • Ergosterol peroxide analogues demonstrated significant anti-proliferation activity against TNBC models.
  • Structure-activity relationship studies provided insights for developing improved analogues.
  • Cellular studies showed ergosterol peroxide accumulates in the cytosol and endoplasmic reticulum (ER).

Conclusions:

  • Ergosterol peroxide and its analogues hold promise as potential therapeutic agents for TNBC.
  • The developed chemical probes are valuable tools for further research into ergosterol peroxide's mechanism of action.
  • Understanding ergosterol peroxide's cellular distribution and SAR is crucial for designing more effective drugs.