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The Parathyroid Glands

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Related Experiment Video

Updated: Jan 25, 2026

Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging

Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging

Published on: August 17, 2022

Parathyroid Tissue Cryopreservation: Does the Storage Time Affect Viability and Functionality?

Annalisa Aiti¹, Martina Rossi², Francesco Alviano²

¹Emilia Romagna Cord Blood Bank, Immunohaematology and Transfusion Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.

Biopreservation and Biobanking

|April 27, 2019

Summary

Parathyroid tissue cryopreservation for autotransplantation is viable long-term. Stored parathyroid tissue (PT) maintains its structure and function, producing parathyroid hormone (PTH) even after five years.

Keywords:

cryopreservation functionality parathyroid cells parathyroid glands viability

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Last Updated: Jan 25, 2026

Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging

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Published on: August 17, 2022

Procurement of Parathyroid Glands from Living Donor Pigs and Ex Vivo Identification

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Published on: August 1, 2025

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Published on: April 9, 2015

Area of Science:

Endocrinology
Surgical Pathology
Tissue Engineering

Background:

Parathyroidectomy for hyperparathyroidism can cause hypoparathyroidism, necessitating parathyroid tissue (PT) autotransplantation.
Cryopreservation allows for delayed PT transplantation, but long-term viability and function post-thaw remain variable.
The impact of cryopreservation protocols and storage duration on PT quality requires further investigation.

Purpose of the Study:

To evaluate the ex vivo viability and function of cryopreserved parathyroid tissue (PT) stored for potential autologous transplantation.
To assess the effects of cryopreservation and storage time on PT morphology, PTH synthesis, and secretion.

Main Methods:

Histological analysis of PT morphology before and after cryopreservation.
Assessment of PTH synthesis and secretion by cryopreserved PT in response to varying calcium concentrations.
In vitro culture of isolated PT cells to evaluate viability and function.

Main Results:

Cryopreserved PT demonstrated preserved histological architecture after storage.
The tissue remained viable and capable of synthesizing and secreting parathyroid hormone (PTH).
These functions were maintained even after up to five years of cryopreservation.

Conclusions:

Cryopreservation is a suitable method for storing parathyroid tissue (PT) for autotransplantation.
Stored PT maintains its viability, histological integrity, and functional capacity to produce PTH long-term.
This supports the use of cryopreserved PT for managing hypoparathyroidism post-parathyroidectomy.